Accession Number : AD1010161


Title :   Targeting Palmitoyl Acyltransferases in Mutant NRAS-Driven Melanoma


Descriptive Note : Technical Report,01 Aug 2013,31 Jul 2015


Corporate Author : Massachusetts General Hospital Boston United States


Personal Author(s) : Wu,Xu


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1010161.pdf


Report Date : 01 Oct 2015


Pagination or Media Count : 31


Abstract : Mutations in N-RAS are linked to 20% of melanoma with no effective treatment. Targeting palmitoyl acyltransferases (PATs) involved in N-RAS regulation could be a novel strategy to treat N-RAS mutant melanoma. The objective of the project is to identify PATs responsible for NRAS activation in melanoma cells using chemical biology and functional genomic approaches. In the first year of the study, we have developed more potent chemical probes to profile PATs in cells, and have carried out PATs profiling in melanoma cells using chemical probes and mRNA profiling. We have identified candidate PATs highly expressed in NRAS melanoma cells. We have developed shRNA reagents to further study their functions.


Descriptors :   mutations , cancer , activation , cells (biology) , ribonucleic acids , Proteomics , mass spectrometry , Histology , proteins , Bioinformatics


Distribution Statement : APPROVED FOR PUBLIC RELEASE