Accession Number : AD1010002


Title :   Improved Ribosome-Footprint and mRNA Measurements Provide Insights into Dynamics and Regulation of Yeast Translation


Descriptive Note : Journal Article - Open Access


Corporate Author : University of Pennsylvania Philadelphia United States


Personal Author(s) : Weinberg,David E ; Shah,Premal ; Eichhorn,Stephen W ; Hussmann,Jeffrey A ; Plotkin,Joshua B ; Bartel,David P


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1010002.pdf


Report Date : 11 Feb 2016


Pagination or Media Count : 17


Abstract : Ribosome-footprint profiling provides genome-wide snapshots of translation, but technical challenges can confound its analysis. Here, we use improved methods to obtain ribosome-footprint profiles and mRNA abundances that more faithfully reflect gene expression in Saccharomyces cerevisiae. Our results support proposals that both the beginning of coding regions and codons matching rare tRNAs are more slowly translated. They also indicate that emergent polypeptides with as few as three basic residues within a ten-residue window tend to slow translation. With the improved mRNA measurements, the variation attributable to translational control in exponentially growing yeast was less than previously reported, and most of this variation could be predicted with a simple model that considered mRNA abundance, upstream open reading frames, cap-proximal structure and nucleotide composition, and lengths of the coding and 5' UTRs. Collectively, our results provide a framework for executing and interpreting ribosome profiling studies and reveal key features of translational control in yeast.


Descriptors :   genomes , cells(biology) , proteins , molecules , gene expression , ribonucleic acids


Distribution Statement : APPROVED FOR PUBLIC RELEASE