Accession Number : AD1009531

Title :   Intracellular Detection of Viral Transcription and Replication Using RNA FISH

Descriptive Note : Technical Report

Corporate Author : USAMRIID Frederick United States

Personal Author(s) : Lindquist,Michael E

Full Text :

Report Date : 26 May 2016

Pagination or Media Count : 6

Abstract : Many hemorrhagic fever viruses require BSL-3 or 4 laboratory containment for study. The necessary safety precautions associated with this work often contribute to longer assay times and lengthy decontamination procedures. In this Chapter, we will discuss recent advances in RNA fluorescence in situ hybridization (FISH) that not only allow entirely new investigations into the replication of these viruses, but also how this method can be applied to any virus with a known sequence and how it can be rapidly performed to minimize time spent in high containment. We have adapted existing protocols for mRNA detection with appropriate changes for examining viruses in a variety of containment laboratories.

Descriptors :   RIBONUCLEIC ACIDS , Hemorrhagic fevers

Distribution Statement : APPROVED FOR PUBLIC RELEASE