Accession Number : AD1009527


Title :   Informing the Historical Record of Experimental Nonhuman Primate Infections with Ebola Virus: Genomic Characterization of USAMRIID Ebola Virus/H.sapiens-tc/COD/1995/Kikwit-9510621 Challenge Stock R4368 and Its Replacement R4415


Descriptive Note : Journal Article


Corporate Author : USAMRIID Frederick United States


Personal Author(s) : Kugelman,Jeffrey R ; Rossi,Cynthia A ; Wiley,Michael R ; Ladner,Jason T ; Nagle,Elyse R ; Pfeffer,Bradley P ; Garcia,Karla ; Prieto,Karla ; Wada,Jiro ; Kuhn,Jens H ; Palacios,Gustavo


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1009527.pdf


Report Date : 20 Mar 2016


Pagination or Media Count : 25


Abstract : The creation of licensed medical countermeasures against Select Agents such as Ebola virus (EBOV) is critically dependent on the use of standardized reagents, assays, and animal models. We performed full genome reconstruction, population genomics, contaminant analysis, and characterization of the glycoprotein gene editing site of historical United States Army Medical Research Institute of Infectious Diseases (USAMRIID) nonhuman-primate challenge stock Ebola virus Kikwit R4368 and its 2014 replacement R4415. We also provide characterization of the master stock used to create R4415. The obtained data are essential to understanding the quality of the seed stock reagents used in pivotal animal studies that have been used to inform medical countermeasure development. Furthermore, these data might add to the understanding of the influence of EBOV variant populations on pathogenesis and disease outcome and inform attempts to avoid the evolution of EBOV escape mutants in response to current therapeutics. Finally, as the primary challenge stocks have changed over time, these data will provide a baseline for understanding and correlating past and future animal study results.


Descriptors :   GLYCOPROTEINS , EBOLA VIRUS , PATHOGENESIS , genomics


Distribution Statement : APPROVED FOR PUBLIC RELEASE