Accession Number : AD1009267


Title :   Correlative Light and Electron Microscopy (CLEM) and its applications in infectious disease


Descriptive Note : Technical Report


Corporate Author : USAMRIID Frederick United States


Personal Author(s) : Koistinen,Keith A ; Blancett,Candace ; Sun,Mei


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1009267.pdf


Report Date : 20 May 2016


Pagination or Media Count : 23


Abstract : Correlative light and electron microscopy (CLEM) is an effective technique used to study biological samples. Signal-specific indicators observable under light microscopy (LM) allow scientists to locate areas of interest for high resolution ultra-structure observation under the electron microscope (EM). Recent method developments provided breakthroughs creating an effective, direct, and accurate research diagnostic tool for functionally related structural biological studies. CLEM is particularly useful in infectious disease research where there is need to study, at nanoscale, objects of interest which are commonly part of rare transient events or afflict a particular cell among a majority of unaffected cells. In this review we discuss several CLEM methods, summarize currently available fluorescent markers, and discuss CLEM instrumentation setups for novel approaches to imaging cellular events in infectious disease.


Descriptors :   ELECTRON MICROSCOPY , electron microscopes , imaging techniques , INFECTIOUS DISEASES


Distribution Statement : APPROVED FOR PUBLIC RELEASE