Accession Number : AD1007743


Title :   The Vibrio cholerae VprA-VprB Two-Component System Controls Virulence Through Endotoxin Modification


Descriptive Note : Journal Article


Corporate Author : University of Texas at Austin Austin United States


Personal Author(s) : Herrera,Carmen M ; Crofts,Alexander A ; Henderson,Jeremy C ; Pingali,S C ; Davies,Bryan W ; Trent,M S


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1007743.pdf


Report Date : 23 Dec 2014


Pagination or Media Count : 17


Abstract : The bacterial cell surface is the first structure the host immune system targets to prevent infection. Cationic antimicrobial peptides of the innate immune system bind to the membrane of Gram-negative pathogens via conserved, surfaceexposed lipopolysaccharide (LPS) molecules. We recently reported that modern strains of the global intestinal pathogen Vibrio cholerae modify the anionic lipid A domain of LPS with a novel moiety, amino acids. Remarkably, glycine or diglycine addition to lipid A alters the surface charge of the bacteria to help evade the cationic antimicrobial peptide polymyxin. However, the regulatory mechanisms of lipid A modification in V. cholerae are unknown. Here, we identify a novel two-component system that regulates lipid A glycine modification by responding to important biological cues associated with pathogenesis, including bile, mildly acidic pH, and cationic antimicrobial peptides. The histidine kinase Vc1319 (VprB) and the response regulator Vc1320 (VprA) respond to these signals and are required for the expression of the almEFG operon that encodes the genes essential for glycine modification of lipid A. Importantly, both the newly identified two-component system and the lipid A modification machinery are required for colonization of the mammalian host. This study demonstrates how V. cholerae uses a previously unknown regulatory network, independent of well-studied V. cholerae virulence factors and regulators, to respond to the host environment and cause infection.


Descriptors :   Virulence , endotoxins , bacteria , cells (biology) , surfaces , peptides , lipids , lipopolysaccharides , amino acids , pathogenesis , phosphorous transferases


Distribution Statement : APPROVED FOR PUBLIC RELEASE