Accession Number : AD1007471


Title :   Engineering of an Extremely Thermostable Alpha/Beta Barrel Scaffold to Serve as a High Affinity Molecular Recognition Element for Use in Sensor Applications


Descriptive Note : Technical Report,01 Aug 2012,31 Jul 2015


Corporate Author : Columbia University New York United States


Personal Author(s) : Banta,Scott ; Haghpanah,Jennifer


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1007471.pdf


Report Date : 23 Dec 2015


Pagination or Media Count : 16


Abstract : The overall goal of the project was to evolve a highly thermostable enzyme (alcohol dehydrogenase D (AdhD) from Pyrococcus furiosus) to bind an explosive molecule, RDX. The enzyme naturally catalyzes the nicotinamide cofactor-dependent oxidation or reduction of alcohols, aldehydes, ketones and carbohydrates. Directed evolution techniques were used to convert the enzyme into a simple binder of the small molecule RDX. Novel binders have been identified and characterized with sub-millimolar dissociation constants. These could be further developed for use in robust and low cost biosensors.


Descriptors :   RDX , explosives detection , biomolecules , evolution , proteins , enzymes , evolution


Distribution Statement : APPROVED FOR PUBLIC RELEASE