Accession Number : AD1007149


Title :   Tribosupplementation with Lubricin in Prevention of Post-Traumatic Arthritis


Descriptive Note : Technical Report,30 Sep 2012,29 Sep 2015


Corporate Author : Rhode Island Hospital Providence United States


Personal Author(s) : Jay,Gregory ; Fleming,Braden


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1007149.pdf


Report Date : 01 Dec 2015


Pagination or Media Count : 14


Abstract : Full length recombinant human lubricin (rhPRG4) maximally reduced friction between apposed explants of bovine articular cartilage. Secretion levels exceeded 1 mg/ml when expressed by transfected CHO-M cells. This level of expression is commercially scalable and will thus enable production of a GMP protein for clinical use. A purification bioprocess utilizing anion exchange, hydroxyapatite and a hydrophobic exchange media resins achieves a high level of purity. Explants of bovine articular cartilage cultured and incubated with IL-1 followed by friction testing showed higher friction than explants not receivingIL-1. The rhPRG4 restored low friction and qualitatively restored native PRG4 expression as determined by immunohistochemistry. Cartilage from the femoral condyle shows variations in IL-1 induced elevated friction. Explants from the weight bearing region show a significant response to exogenous rhPRG4 in friction reduction from this simulated inflammatory environment. A large animal trial is now planned to test pre-GMP equivalent rhPRG4 in restoring chondroprotection in the ACL-injured joint.


Descriptors :   arthritis , lubrication , knee , knee injuries , CARTILAGE DISEASES , in vitro analysis


Distribution Statement : APPROVED FOR PUBLIC RELEASE