Accession Number : AD1006130

Title :   Disinfection of Vegetative Cells of Bacillus anthracis

Descriptive Note : Technical Report,01 Nov 2012,31 Oct 2014

Corporate Author : Edgewood Chemical Biological Center APG United States

Personal Author(s) : Smith,Lisa S ; Rastogi,Vipin K ; Ziemski,Michelle R

Full Text :

Report Date : 01 Mar 2016

Pagination or Media Count : 26

Abstract : Disinfection kinetics of vegetative cells of Bacillus anthracis in water with free available chlorine ([FAC] 2 mg/L) and monochloramine ([MC] 2 mg/L) were established in this study. FAC disinfection was performed in chlorine demand-free phosphate buffer at pH 7 and 8 at two temperatures (5 and 25 deg. C). MC disinfection was performed in normal phosphate buffer at pH 8 at both temperatures. FAC was more effective than MC in causing cell death, which was more rapid at 25 deg. C than at 5 deg. C at both pH 7 and 8. For MC disinfection, the cell inactivation rate was more rapid at 2 deg. C. The disinfection kinetics were rapid within the first 5 min, followed by a slow cell inactivation. The results were complicated by the varying number of spores present in the test inoculums. Although protocols were developed and implemented to minimize the spore number, varying numbers of spores were observed in the different runs. The presence of spores also resulted in high variability,especially at sublethal exposure levels. Our efforts were focused on developing a new culture recipe (RVLS) to ensure aminimal number of spores and to confirm the absence of spores in the sporulation-minus strain (spo-) of B. anthracis.

Descriptors :   cells(biology) , bacillus anthracis , Drinking water , chlorine , spores

Distribution Statement : APPROVED FOR PUBLIC RELEASE