Accession Number : AD1002913

Title :   Toward an Inexpensive Test for Vitamin D Levels in Blood

Descriptive Note : Technical Report,01 Oct 2012,30 Sep 2014

Corporate Author : The Trustees of Columbia University in the City of New York New York United States

Personal Author(s) : West ,Alan C ; Banta,Scott ; Linday,Linda ; Ozbakir,Harun F ; Sambade,David A ; Majumdar,Sudipta

Full Text :

Report Date : 01 Dec 2014

Pagination or Media Count : 19

Abstract : The objective of the research is to test the feasibility of a vitamin D biosensor based on a selective redox enzyme immobilized onto an electrode. In long-term, this will make it possible to create a technology akin to commercial glucose sensors for in-clinic use as part of routine examinations by both reducing the cost of testing vitamin D levels and reducing the time for testing to less than a few minutes. For this purpose, a synthetic gene for recombinant human CYP27B1 (the enzyme which is involved in vitamin D metabolism) was designed. The enzyme was expressed in E. coli and the activity of this enzyme was verified spectrophotometrically and using liquid chromatography-mass spectrometry (LC-MS/MS). Various synthetic redox mediators were tried and cobalt sepulchrate trichloride (Co(sep)3+/2+) was found to enhance the direct electron transfer between electrode and redox center of CYP27B1. This is important for introducing a way to electrochemically measure 25(OH)D (circulating form of vitamin D in blood) levels.

Descriptors :   VITAMIN D

Distribution Statement : APPROVED FOR PUBLIC RELEASE