Accession Number : AD1001844

Title :   Spider Silk: From Protein-Rich Gland Fluids to Diverse Biopolymer Fibers

Descriptive Note : Technical Report,01 Dec 2013,30 Nov 2015

Corporate Author : Arizona State University Tempe United States

Personal Author(s) : Holland,Gregory P ; Yarger,Jeffery L

Full Text :

Report Date : 06 Jan 2016

Pagination or Media Count : 34

Abstract : The primary objective of this research is to elucidate the interactions, mechanisms and biochemistry of the spider silk producing process at the molecular level. Our primary focus is to characterize the protein-rich fluid in the various spider silk producing glands. We have been using a battery of magnetic resonance methods including solution and solid-state nuclear magnetic resonance (NMR) and micro imaging (MRI) in combination with wide angle and small angle X-ray diffraction (WAXD and SAXD) techniques at Argonne National Laboratory (ANL) to probe silk protein structure and dynamics prior to and following fiber formation. We have established a number of methods for isotopically (2H/13C/15N) enriching the silk proteins during the course of our AFOSR funding that have allowed us to investigate the structure, dynamics, and organization of spider silk protein within the silk gland and in the final spun fiber with a range of magnetic resonance methods. We successfully developed magnetic resonance imaging (MRI) techniques with localized spectroscopy to probe the silk glands of spiders and map protein structure through out the silk gland.

Descriptors :   SPIDERS , silk , MILITARY APPLICATIONS , magnetic resonance imaging

Distribution Statement : APPROVED FOR PUBLIC RELEASE