Accession Number : AD1001193

Title :   The Parkinson Transcriptome Project: Roadmap to Personalized Care

Descriptive Note : Technical Report

Corporate Author : The Brigham and Womens Hospital, Inc Boston United States

Personal Author(s) : Scherzer,Clemens R

Full Text :

Report Date : 01 Aug 2015

Pagination or Media Count : 26

Abstract : This study provides a comprehensive catalog of gene expression - both protein-coding and non-coding - specifically indopamine neurons in situ in human brain. Going beyond traditional messenger RNA sequencing, all polyadenylated and nonpolyadenylatedtranscripts over 50 bases were deeply sequenced using ribo-depleted RNA from dopamine neurons lasercapturedfrom human post-mortem brains of patients with Parkinsons disease using laser-capture RNA sequencing. We showunequivocally that non-coding RNAs are abundantly transcribed in human dopamine neurons and highlight significant changesin the expression of both protein-coding and non-coding transcripts in patients with clinical and neuropathological Parkinsonsdisease. Expression Quantitative Trait Locus analysis indicates that the expression of many non-coding and protein-codingtranscripts in nigral dopamine neurons is associated with genetic variants. Finally, these pilot phase data support the feasibilityof measuring select RNAs linked to Parkinsons in vulnerable dopamine neurons as possible biomarkers in blood.

Distribution Statement : APPROVED FOR PUBLIC RELEASE