Accession Number : AD0712613


Title :   ROLE OF INTERFERON IN THE PROPAGATION OF MM VIRUS IN L CELLS


Corporate Author : SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TX


Personal Author(s) : Giron, David J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/712613.pdf


Report Date : 18 Aug 1969


Pagination or Media Count : 8


Abstract : MM virus propagated in mouse brain replicates to low titers in L cells without production of cytopathic effect (CPE). After growing the virus in BHK-21 cells, however, the virus replicates to high titers in L cells with complete CPE. It was found that suspensions of MM virus propagated in L cells directly from the mouse brain contained much more interferon than did suspensions of virus which had first been grown in BHK-21 cells. Mouse brain suspensions of the virus were also found to contain high interferon titers. Treatment of L cells with actinomycin D before infection with mouse brain-grown virus resulted in full virus replication with CPE. BHK-21 cell-grown virus diluted in L cell interferon behaved like mouse brain-grown virus in L cells. It is concluded that the presence of interferon in the inoculum is largely responsible for the suppression of MM virus replication in L cells.


Descriptors :   *ARBOVIRUSES , *TISSUE CULTURE CELLS , ANTIBIOTICS , BRAIN , CELLS(BIOLOGY) , GROWTH(PHYSIOLOGY) , MICE , PATHOLOGY


Subject Categories : Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE