Accession Number : AD0479136


Title :   INHIBITION OF PROTEIN SYNTHESIS BY SPERMINE IN GROWING CELLS OF STAPHYLOCOCCUS AUREUS


Descriptive Note : Technical manu.


Corporate Author : ARMY BIOLOGICAL DEFENSE RESEARCH CENTERFREDERICK MD


Personal Author(s) : Friedman, Mischa E ; Bachrach, Uriel


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/479136.pdf


Report Date : Feb 1966


Pagination or Media Count : 22


Abstract : Staphylococcus aureus SFL 9725 incorporated valine-C14 into cellular protein. This incorporation was inhibited by spermine when the pH of the culture was, adjusted to 7.8 and the inhibition was antagonized partially by Mg(++). The incorporation of C14 labeled leucine, phenylalanine, lysine, arginine, and possibly glutamic acid was inhibited to a much greater extent than that of alanine, glycine, or threonine. The uptake of spermine-C14 by S. aureus cells was rapid. More than 50% of the radioactivity resided in the soluble extract. The protein fraction of the soluble extract contained 99% of the label, whereas the ribonucleic acid and deoxyribonucleic acid fractiosn contained little or no spermine-C14.


Descriptors :   *PROTEINS , *ANTIMETABOLITES , IONS , MAGNESIUM , DEOXYRIBONUCLEIC ACIDS , INHIBITION , RIBONUCLEIC ACIDS , PH FACTOR , AMINO ACIDS , LABELED SUBSTANCES , RADIOACTIVE ISOTOPES , GROWTH SUBSTANCES , BASES(CHEMISTRY) , STAPHYLOCOCCUS AUREUS


Subject Categories : Biochemistry


Distribution Statement : APPROVED FOR PUBLIC RELEASE