Accession Number : AD0476396


Title :   EFFECT OF FORMALIN ON THE IMMUNOCHEMICAL AND BIOLOGICAL ACTIVITY OF STAPHYLOCOCCAL ENTEROTOXIN


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Silverman, Sidney J ; Schantz, Edward J ; Espeseth, David A ; Roessler, William G


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/476396.pdf


Report Date : Dec 1965


Pagination or Media Count : 13


Abstract : Purified staphylococcal enterotoxin B was treated with formalin at 37 C and pH 7.3. The effect of formalin concentration and duration of exposure was studied by immunochemical and biological means. By 8 hours, toxin treated with 0.15 or 0.30% formalin (v/v) lost some of its ability to precipitate antibody. At 48 hours the toxoid behaved as a cross-reacting antigen in that an antibody excess occurred throughout the range of the quantitative precipitin titrations and the zone of antibody excess was followed by a zone in which both antigen and antibody were in excess. In the Ouchterlony double gel diffusion test after 48 hours, toxoid gave a reaction of partial identity when compared with toxin. When tested by immunoelectrophoresis at pH 6.0, 0.1 M PO4 buffer, the formalin- treated toxin migrated further toward the cathode than did the toxin.


Descriptors :   *TOXINS AND ANTITOXINS , *STAPHYLOCOCCUS , BIOCHEMISTRY , ANTIBODIES , INHIBITION , ANTIGENS , ELECTROPHORESIS , FORMALDEHYDE , BIOASSAY , MONKEYS


Subject Categories : Biochemistry
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE