Accession Number : AD0472299


Title :   BACILLUS SUBTILIS PHAGE SP-10: CONCURRENT CHANGES IN TRANSDUCING EFFICIENCY AND CONTENT OF TRANSFORMING DEOXYRIBONUCLEIC ACID,


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Taylor, Martha J ; Thorne, Curtis B


Report Date : Sep 1965


Pagination or Media Count : 24


Abstract : Spores of Bacillus subtilis infected with transducing phage SP-10 served as convenient inocula for broth cultures from which transducing phage was harvested. Methods are described for producing highly infected spores. The inoculum level of infected spores in nutrient broth yeast-extract glucose medium affected the transducing efficiency of SP-10 in lysates of these cultures. The combination of inoculum level and incubation time required to produce the best transducing phage had to be determined empirically for each batch of infected spores. Several possible explanations for the difference between lysates having high (HTE) and those having low (LTE) transducing efficiency were ruled out by special experiments. The hypothesis is presented that some cultural condition resulting from a relatively low inoculum of phage-infected spores favors the incorporation by phage particles of bacterial deoxyribonucleic acid (DNA) in the manner required for the production of transducing phage. Support for this hypothesis is a demonstration, through transformation experiments with DNA extracted from HTE and LTE phage particles, that populations of HTE phage particles yielded significantly more (7 to 27 times) transforming activity per microgram of DNA than did populations of LTE phage. (Author)


Descriptors :   *BACILLUS SUBTILIS , BACILLUS , CULTURE MEDIA , SPORES , INFECTIOUS DISEASES , PHYSIOLOGY , DEOXYRIBONUCLEIC ACIDS , BIOCHEMISTRY


Distribution Statement : APPROVED FOR PUBLIC RELEASE