Accession Number : AD0467170


Title :   INFLUENCE OF DEUTERIUM OXIDE ON BIOLOGICAL SYSTEMS: A. VIRUS GROWTH IN TISSUE CULTURE CELLS. B. OXIDATION OF CHOLESTEROL BY RAT LIVER MITOCHONDRIA.


Descriptive Note : Progress rept. Jun 64-May 65,


Corporate Author : PENNSYLVANIA UNIV PHILADELPHIA WISTAR INST


Personal Author(s) : Kritchevsky, David


Report Date : 01 Jul 1965


Pagination or Media Count : 11


Abstract : The purpose of this research was to investigate the effects of deuterium oxide (D20) upon virus growth in tissue culture cell systems. The work carried out this year was aimed at examining the possibility that the enhancement of virus growth observed with poliomyelitis virus was characteristic of all RNA containing viruses. Three RNA viruses, of varying size, were studied. The first was Western Equine Encephalitis 9wEE) which was propogated in chick embryo fibroblasts grown in Petri dishes. The overlay medium contained 25% D2O and virus growth was assayed by measuring plaque size. In this experiment D2O exerted an inhibitory effect of the growth of WEE virus. A second series of experiments were carried out with Rubells virus which was grown in monolayer cultures of monkey or rabbit kidney. The medium contained 10 or 50% of D2O. Virus growth was assayed by the extent of interference of the virus with the cytopathic effect of ECHO virus II. Presence of D2O in the medium exerted a slight enhancing effect upon the yield of the intra- and extracellular virus. The effect is not considered significant. In a third series of experiments, the effect of D2O upon the growth of several strains of rabies virus in a variety of tissue culture culture cell systems was studied. Growth of rabies virus was quantitated by a fluoroscent antibody technique as well as by titration in mich. The presence of D2O (25 or 40%) in the medium did not affect virus replication in any case. (Author)


Descriptors :   *DEUTERIUM COMPOUNDS , METABOLISM , HEAVY WATER , TISSUE CULTURE CELLS , GROWTH(PHYSIOLOGY) , WESTERN EQUINE ENCEPHALOMYELITIS VIRUS , RUBELLA , ECHOVIRUSES , RABIES VIRUS , ENZYMES , CHOLESTEROL , MITOCHONDRIA , OXIDATION , KIDNEYS , LIVER


Distribution Statement : APPROVED FOR PUBLIC RELEASE