Accession Number : AD0466947


Title :   EFFECT OF ENZYMATIC FUNCTIONAL GROUP INHIBITORS ON THE ESTERASES OF CROTALUS ATROX VENOM,


Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY


Personal Author(s) : Brown, John H


Report Date : 23 Apr 1965


Pagination or Media Count : 10


Abstract : The activities of phosphodiesterase, phosphomonoesterase and esterase in Crotalus atrox venom can be completely inhibited by one or more of the following enzymatic functional groupspecific reagents: 2-mercaptoethanol, 2,3dimercaptopropanol, p-hydroxymercuibenzoate, iodoacetamide, diisopropylfluorophosphate, ethylenediaminetetraacetic acid, N-bromosuccinimide. Phosphodiesterase was activated by the divalent cations of iron, cobalt, nickel, manganese, zinc and the alkaline earths; phosphomonoesterase was activated by calcium, iron, cobalt and zinc; esterase was not activated by metal ions. In general, the divalent cations of cadmium, mercury and copper inhibited all three enzymes. (Author)


Descriptors :   *ENZYMES , BIOCHEMISTRY , VENOMS , ORGANIC PHOSPHORUS COMPOUNDS , REPTILES , CARBOXYLIC ESTER HYDROLASES , PHOSPHORYLATION , TOXICITY , PH FACTOR , HYDROLYSIS , ORGANOMETALLIC COMPOUNDS , IONS , ALKALINE EARTH METALS


Distribution Statement : APPROVED FOR PUBLIC RELEASE