Accession Number : AD0466292


Title :   TO STUDY THERAPEUTIC PROPERTIES OF BLOOD PLASMA IN THE THERAPY OF TOURNIQUET SHOCK.


Descriptive Note : Progress rept. 1 May 64-15 Jul 65,


Corporate Author : JEWISH HOSPITAL ST LOUIS MO


Personal Author(s) : Pareira, Morton D


Report Date : 15 Jul 1965


Pagination or Media Count : 21


Abstract : Tests of therapeutic effectiveness of infusions of different human plasma fractions in the treatment of rat tourniquet trauma revealed a striking superiority of Cohn Fraction II in protecting against mortality of the trauma. The substitution of this human plasma fraction for a portion of the rat's own plasma protein in rat plasma markedly improved the therapeutic effectiveness of the rat plasma. Studies of hematocrits and fluid loss into the traumatized hind limbs revealed that animals treated with Fraction II infusions maintained a larger circulating plasma volume (lower hematocrit) than animals infused with albumin or saline. This was thought to be due to recruitment of fluid into the circulating volume combined with a smaller loss into the hind limbs. Chloramphenicol pretreatment of saline-treated traumatized animals did not product any improvement in survival over the appropriate control group. Although infusion of recently collected human plasma did not allow any survival when used to treat standard trauma, gamma globulin from ''fresh'' plasma protected as well as did gamma globulin from aged plasma. This finding suggests that the improved protection found with aged plasma was related to the disappearance of deleterious properties from fresh plasma not associated with the gamma globulin. (Author)


Descriptors :   BLOOD PLASMA , SHOCK THERAPY , AGING(PHYSIOLOGY) , BLOOD PROTEINS , SHOCK(PATHOLOGY) , GAMMA GLOBULIN , RATS , MEDICAL RESEARCH , BIOCHEMISTRY , SURVIVAL(PERSONNEL)


Distribution Statement : APPROVED FOR PUBLIC RELEASE