Accession Number : AD0465855


Title :   ABSCOPAL AND DIRECT EFFECTS OF WHOLE-BODY X-IRRADIATION: RAT KIDNEY MITOTIC ACTIVITY AND DNA CONTENT AFTER UNINEPHRECTOMY,


Corporate Author : NAVAL RADIOLOGICAL DEFENSE LAB SAN FRANCISCO CA


Personal Author(s) : Wachtel, Louis W ; Cole, Leonard J ; Rosen, Victor J , Jr


Report Date : 24 May 1965


Pagination or Media Count : 24


Abstract : The effect on kidneys of X radiation at doses ranging from 500 rad to 4000 rad and measured in unilaterally nephrectomized weanling (24-25 days old) and yound adult (80 days old) rats. Kidney mitotic activity 2-3 days following uninephrectomy was compared with subsequent increases in kidney weight and DNA content. An abscopal (indirect) effect on kidney mitoses was found in unilaterally nephrectomized weanling rats receiving 500 rad total-body X irradiation, but whose kidneys were lead-shielded during irradiation. With the remainder of the body lead-shielded, increasing doses of X radiation directly to the kidneys of unilaterally nephrectomized weanling rats resulted in decreasing mitotic activities: 0 rad = 129-142; 1000 rad = 58-97; 2000 rad = 37-40; 4000 rad = 17-24 mitoses/50 hpf. Three weeks after nephrectomy no difference in kidney weight or DNA content was noted between kidneys irradiated with 1000 rad and the nonirradiated controls. Nonirradiated weanling rats food-deprived for 1-4 days maintained a constant ratio of kidney weight: body surface area despite a large reduction in body weight. No effect on kidney mitoses was found in young adult rats whose kidneys were irradiated with 690 rad at 2 1/2 hours after uninephrectomy. It is concluded that mitotic activity determined 2-3 days after unilateral nephrectomy does not provide a quantitative indicator of subsequent kidney weight or DNA content in X irradiated weanling rats. (Author)


Descriptors :   RADIATION EFFECTS , MITOSIS , EXCISION , DEOXYRIBONUCLEIC ACIDS , RATS , WHOLE BODY IRRADIATION , EXPERIMENTAL DATA , STARVATION


Distribution Statement : APPROVED FOR PUBLIC RELEASE