Accession Number : AD0464210


Title :   PSITTACOSIS GROUP VACCINE PREPARED IN A HUMAN DIPLOID CELL STRAIN


Descriptive Note : Technnical manuscript


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Duff, James T


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/464210.pdf


Report Date : May 1965


Pagination or Media Count : 17


Abstract : Growth characteristics of three agents of the psittacosis group in the L cell line and in human diploid cell strains have been described previously. Applications of these methods of cultivation to preparation of improved psittacosis group vaccines have been investigated. The Borg strain of human pneumonitis agent was used to infect cell cultures of human diploid lung strain, WI-38. Eagle basal medium (BME), which contained 3% calf serum, was removed from infected cell monolayers after 18 hours' incubation at 37 C, and replaced with BME (without serum) or BME containing 0.5% bovine serum albumin. Supernatant fluids were collected after 2 to 3 days at 37 C, and formalin inactivation rates were investigated. The conditions selected for routine inactivation were 0.05% formalin, pH 7.0 to 7.5, and 20 C. Mice injected intraperitoneally with single or multiple injections of vaccine and challenged by the same route at various time intervals after the last injection withstood large doses of homologous active agent. The immunized mice also were protected against a lethal challenge with the heterologous 6BC strain of the psittacosis agent. Cross-protection was further evidenced by protection against the Borg strain using a formalin-inactivated 6BC vaccine.


Descriptors :   *VACCINES , *MIYAGAWANELLA PSITTACI , TISSUE CULTURE CELLS , PREPARATION , HUMANS , LUNG , MICE , IMMUNOLOGY , CULTURE MEDIA , TISSUE CULTURE


Subject Categories : Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE