Accession Number : AD0462472


Title :   TRANSFER OF F-LAC EPISOME TO PASTEURELLA PSEUDOTUBERCULOSIS


Descriptive Note : Technical manuscript


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Lawton, William D ; Zsigray, Robert M ; Surgalla, Michael J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/462472.pdf


Report Date : Apr 1965


Pagination or Media Count : 14


Abstract : The F-lac episome was transferred from Escherichia coli to Pasteurella pseudotuberculosis. The parent P. pseudotuberculosis fermented glycerol, rhamnose, salicin, and xylose, produced urease, VW antigens, and glucose-6-phosphate dehydrogenase, was pigmented, pesticin-sensitive, and virulent, and had a calcium requirement at 37 deg C. Some of the P. pseudotuberculosis F-lac recipients were negative for all of these properties, but became positive for all of them again when the F-lac episome was lost. It appeared that the F-lac carried with it a genetic unit that could repress the expression of several seemingly unrelated properties, and that this repression was removed when the episome was lost.


Descriptors :   *GENETICS , *YERSINIA PSEUDOTUBERCULOSIS , METABOLISM , ESCHERICHIA COLI , INHIBITION , ALCOHOLS , CULTURE MEDIA , LACTOSE , GLYCOSIDES , UREASE , PENTOSES , XYLOSE


Subject Categories : Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE