Accession Number : AD0460633


Title :   TECHNIQUE FOR THE STUDY OF BACTERIAL PERMEABILITY USING A STOPPED-FLOW RAPID MIXING APPARATUS,


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Lovett, Stanley


Report Date : Mar 1965


Pagination or Media Count : 17


Abstract : A technique is described that enables the rapid changes in size and refractive index of bacteria to be determined after the bacteria are introduced into media of different osmotic strength. This is accomplished by measuring the change in intensity of light scattered at 90 degrees to a beam of light passing through a suspension of bacteria that have been efficiently mixed with a medium having a different osmotic strength. The flow of the mixed liquids is stopped rapidly and observations of the scattered light are made from approximately 1.5 milliseconds after mixing. Curves are presented showing the time course of the osmotic filtration of water into and out of cells of Serratia marcescens obtained when the cells are introduced into solutions of different concentrations of sodium chloride. The technique is extended to the study of rapidly permeating solutes, e.g. glycerol and deuterium oxide, and to more slowly permeating solutes, e.g. urea. (Author)


Descriptors :   *SERRATIA MARCESCENS , PERMEABILITY , CULTURE MEDIA , OSMOSIS , GROWTH(PHYSIOLOGY) , REFRACTIVE INDEX , SODIUM COMPOUNDS , CHLORIDES , SOLUTIONS(MIXTURES) , MEASUREMENT , INSTRUMENTATION , TEST METHODS


Distribution Statement : APPROVED FOR PUBLIC RELEASE