Accession Number : AD0459388


Title :   ATTENUATED TOTAL REFLECTANCE IN INFRARED SPECTROPHOTOMETRY OF BIOLOGICAL SYSTEMS


Descriptive Note : Technical manuscript


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Wagman, Jack ; Weneck, Edward J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/459388.pdf


Report Date : Mar 1965


Pagination or Media Count : 21


Abstract : The attenuated total reflectance (ATR) method for obtaining infrared absorption spectra has been shown to be a feasible technique for the study of biological and other materials in their normal aqueous environment. Water-compensated ATR spectra for a number of simple compounds, a protein (bovine serum albumin), and a bacterium (Serratia marcescens) in water solution or suspension, were generally similar to normal transmission spectra for dried samples; but showed some distinctive features not present in the latter. Some limitations of the method and steps taken to overcome them are discussed.


Descriptors :   *INFRARED SPECTROSCOPY , *BACTERIA , *PROTEINS , SOLUTIONS(MIXTURES) , INFRARED SPECTRA , KETONES , SERUM ALBUMIN , SPECTROPHOTOMETERS , SERRATIA MARCESCENS


Subject Categories : Biochemistry
      Atomic and Molecular Physics and Spectroscopy


Distribution Statement : APPROVED FOR PUBLIC RELEASE