Accession Number : AD0456335


Title :   NEW TECHNIQUE FOR STUDYING THE INTRAPHAGOCYTE FATE OF BACTERIA,


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Janssen, Werner A ; Surgalla, Michael J


Report Date : Jan 1965


Pagination or Media Count : 10


Abstract : A technique for the direct microscopic observation and quantitation of survival of Pasteurella pestis following ingestion by phagocytes in guinea pig peritoneal exudate in vivo is described. Infected phagocytes were removed from the animals at intervals following inoculation, and diluted in appropriate bacteriological media. Microcultures were prepared for observation under the phase microscope. Growth of virulent and avirulent P. pestis within the phagocytes was initiated many hours after ingestion. Without the use of bacteriological media, intraphagocyte organisms grew poorly or not at all in vitro, and visualization of the exudate preparations using phase microscopy was difficult. Initiation of growth in neutrophiles was much less frequent than in microphages. Growth in macrophages often occurred after initial observation of conventionally stained preparations had failed to reveal intracellular organisms. The technique provided further evidence that the phagocytic defense system of guinea pigs is ineffective against P. pestis. (Author)


Descriptors :   *YERSINIA PESTIS , *CELLS(BIOLOGY) , CULTURE MEDIA , PHYSIOLOGY , LEUKOCYTES , GROWTH(PHYSIOLOGY) , MICROSCOPY , GUINEA PIGS , RETICULOENDOTHELIAL SYSTEM


Distribution Statement : APPROVED FOR PUBLIC RELEASE