Accession Number : AD0455886


Title :   PARTIAL PURIFICATION OF THE ORGANISM OF PSITTACOSIS GROWN IN CHICK EMBRYO YOLK SAC


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Shirey, William N ; Dorsey, Warren G ; Patrick, William C , III


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/455886.pdf


Report Date : Dec 1964


Pagination or Media Count : 16


Abstract : A procedure described for the partial purification of suspensions of the organism of psittacosis utilizes tryptic digestion, calcium chloridedextran sulfate precipation, and centrifugation through a sucrose barrier. More than 50% of the organisms in the original suspension are recovered in 80% of the original volume with approximately 95% of the lipids and 85% of the proteins removed. This method is suggested as a preliminary purification procedure in laboratories desiring relatively large amounts of purified agent for studies on metabolism, physical properties, or antigenicity of the organism. Significant aspects of the investigations leading to the selection of the procedure are included.


Descriptors :   *CULTURE MEDIA , *MIYAGAWANELLA PSITTACI , METABOLISM , PURIFICATION , GROWTH(PHYSIOLOGY)


Subject Categories : Microbiology
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE