Accession Number : AD0455883


Title :   PHOTOSENSITIZATION OF ARBOVIRUSES BY ACRIDINE DYES


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Officer, Julius E ; Hoffman, Edwin J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/455883.pdf


Report Date : Dec 1964


Pagination or Media Count : 15


Abstract : This paper describes the photodynamic action of proflavine and acridine orange on eastern equine encephalitis (EEE) and Venezuelan equine encephalitis (VEE) viruses. The viruses were more sensitive to the photodynamic action of acridine orange than to that of proflavine. VEE virus was slightly more resistant than EEE virus to the photodynamic action of either dye. The dye- virus complex proved to be irreversible in that the dyetreated virus remained photosensitive upon further dilution or when the excess dye was removed by dialysis or centrifugation. Supernatant fluids of actively growing cell cultures partially protected the photosensitive virus from photoinactivation. This prevented the use of photodynamic action that would differentially separate parent virus from newly synthesized progeny as has been accomplished with other viruses. The addition of reducing compounds, cysteine, glutathione, or thiourea, also protected the photosensitive virus against inactivation by light somewhat. The photoinavtivation rate of infectious nucleic acid, photosensitized by these dyes, was appreciably less than that for whole virus.


Descriptors :   *PHOTOSENSITIVITY , *ARBOVIRUSES , *BIOLOGICAL STAINS , INHIBITION , LIPOPROTEINS , RIBONUCLEIC ACIDS , AMINO ACIDS , NUCLEIC ACIDS , BIOASSAY , GLUTATHIONE , VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS


Subject Categories : Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE