Accession Number : AD0453399


Title :   RELATIONSHIP BETWEEN COMPOUND STRUCTURE AND INFECTIVITY EFFECT ON EEE VIRUS


Descriptive Note : Technical manuscript


Corporate Author : VICKERS-ARMSTRONGS (AIRCRAFT) LTD WEYBRIDGE (UNITED KINGDOM)


Personal Author(s) : Wachter, Ralph F ; Comer, JoAnn F


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/453399.pdf


Report Date : Sep 1964


Pagination or Media Count : 15


Abstract : A number of sulfhydryl (-SH) compounds and the nonsulfur analogs of some of these compounds were tested for their effect on the infectivity of partially purified eastern equine encephalitis virus during storage at 4 C. Thiourea was found to be the most effective stabilizer; whereas urea and guanidine caused a loss of viral infectivity. Thiosemicarbazide and semicarbazide showed effects similar to those of thiourea and urea. Viral inactivation produced by p- chloromercuribenzoate, sodium thioglycollate, or sodium ascorbate could be prevented by thiourea. No correlation was found between the oxidation-reduction potential of virus suspensions containing the various compounds and the infectivity titer, or change in titer, of these suspensions. The presence of an -SH group in the molecule is not a requisite for stabilization: glutamine and dipyridyl exhibited good stabilizing properties.


Descriptors :   *EASTERN EQUINE ENCEPHALOMYELITIS VIRUS , BIOCHEMISTRY , METABOLISM , VIABILITY , MOLECULAR STRUCTURE , PHYSIOLOGY , INHIBITION , ANALOG SYSTEMS , PH FACTOR , SULFUR COMPOUNDS , ASCORBIC ACID , GLUTAMINE , THIOUREA


Subject Categories : Microbiology
      Medicine and Medical Research
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE