Accession Number : AD0453395


Title :   A QUANTITATIVE ASSAY FOR CRUDE ANTHRAX TOXINS


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Haines, Bertram W ; Klein, Frederick ; Lincoln, Ralph E


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/453395.pdf


Report Date : Oct 1964


Pagination or Media Count : 30


Abstract : The whole crude toxins of Bacillus anthracis, although apparently responsible for the death of animals with anthrax, had never been quantitated. A total of 14 lots of the toxic culture filtrate of B. anthracis were pooled into one large lot of crude anthrax toxins. An extensive assay of this reference material was conducted in four laboratories using the time-to-death of the IV-challenged Fischer 344 rat as the response variable. Doses of the material were varied by concentration/dilution and by volume. The data from this study were used to define a potency unit of the crude anthrax toxins. Procedures were developed and illustrated for the assay of unknown lots of the toxins by comparing the rat time-to-death response to the unknown with either (1) the responses reported in this study or (2) directly with the rat responses to a new sample of the reference toxins. The possibilities and limitations of this standardization and of the statistical procedure through which it was developed are discussed.


Descriptors :   *BACTERIAL TOXINS , *BACILLUS ANTHRACIS , EXPERIMENTAL DATA , RATS , TEST METHODS , ANALYSIS OF VARIANCE , METABOLISM , LETHAL DOSAGE , BIOASSAY


Subject Categories : Toxicology


Distribution Statement : APPROVED FOR PUBLIC RELEASE