Accession Number : AD0452980


Title :   GROWTH OF ANIMAL CELLS SUSPENDED IN SERUM-FREE MEDIA. 2. FERMENTOR CULTURES


Descriptive Note : Technical Manuscript


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Nagle, Stanley C , Jr ; Brown, Bruce L ; Taylor, Gordon W


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/452980.pdf


Report Date : Oct 1964


Pagination or Media Count : 12


Abstract : Growth of cat kidney, HeLa, and L cells was obtained in 4-liter volumes of a chemically defined medium in 7.5-liter fermentors. Viable cell counts of 23 x 10 to the 8th power to 33 x 10 to the 8th power per culture were recorded after 11 to 13 days of incubation from inocula of 1 x 10 to the 8th power to 4 x 10 to the 8th power cells. Under similar conditions, cultures of L cells in serum-free lactalbumin hydrolyzate medium yielded 34 x 10 to the 8th power viable cells in 7 days. L cells grown in 14-liter fermentors containing 10 liters of serum-free lactalbumin hydrolyzate medium yielded a total viable count of 14 x 10 to the 9th power cells (2 tanks) from an inoculum of 56 x 10 to the 7th power cells after 16 days of incubation.


Descriptors :   *CULTURE MEDIA , *TISSUE CULTURE , LIVER , FERMENTATION , BLOOD SERUM , NUTRITION , KIDNEYS , CATS


Subject Categories : Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE