Accession Number : AD0452977


Title :   A NEW METHOD FOR PURIFYING COXIELLA BURNETII BY DEXTRAN SULFATE PRECIPITATION


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Davis, Jack L ; Patrick, William C , III


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/452977.pdf


Report Date : Oct 1964


Pagination or Media Count : 16


Abstract : This paper describes a simple method for purifying Coxiella burnetii cultivated in chick embryos. The procedure is based upon the preliminary clarification of infected whole-egg suspensions by precipitating the low-density lipoproteins and other extraneous solids using dextran sulfate, calcium chloride, and sodium chloride solutions, then concentrating the rickettsiae by centrifugation.


Descriptors :   *COXIELLA BURNETII , *PRECIPITATION , MICROSCOPY , VISUAL INSPECTION , INFECTIOUS DISEASES , ANTIGENS , BIOASSAY , GUINEA PIGS , DEXTRAN , BIOLOGY , ANTIBODIES


Subject Categories : Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE