Accession Number : AD0451521


Title :   THE SYNTHESIS OF BETA-GALACTO- AND BETA-GLUCOPYRANOSYL DISACCHARIDES BY SPOROBOLOMYCES SINGULARIS,


Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB


Personal Author(s) : Gorin,P A J ; Spencer,J F T ; Phaff,H J


Report Date : 01 Jun 1964


Pagination or Media Count : 11


Abstract : Synthesis of beta-D-galactopyranosyl disaccharides takes place in growing cultures of Sporobolomyces singularis containing lactose and a sugar acceptor. The products of galactosyl transfer using various acceptors arise mainly from substitution of secondary rather than primary hydroxyl groups. The minimal structural requirement for reaction with the acceptor appears to be a hydroxyl vicinal to the substituted hydroxyl group. A mechanism that accounts for the formation of secondary disaccharides is postulated. The organism also contains a beta-glucosidase which transfer Obeta-D-glucopyranosyl groups from cellobiose to sugar acceptors. The positions of the glycosidic linkages in the disaccharide products are identical with those formed in the corresponding galactosyl transfer reaction. (Author)


Descriptors :   DISACCHARIDES , CULTURE MEDIA , MONOSACCHARIDES , LACTOSE , TRISACCHARIDES , PENTOSES , XYLOSE , GLUCOSE , CHROMATOGRAPHY , CANADA


Distribution Statement : APPROVED FOR PUBLIC RELEASE