Accession Number : AD0451382


Title :   FLUORESCENT ANTIBODY TEST FOR THE SERODIAGNOSIS OF VISCERAL LEISHMANIASIS


Corporate Author : WALTER REED ARMY INST OF RESEARCH WASHINGTON DC


Personal Author(s) : Duxbury, Ralph E ; Sadun, Elvio H


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/451382.pdf


Report Date : Jan 1964


Pagination or Media Count : 6


Abstract : The indirect fluorescein-labeled antibody technic was used and evaluated in the serodiagnosis of kala-azar in humans. The tests were performed on slides using Leishmania donovani leptomonad forms as antigen. Evans blue, employed as a counter-stain, resulted in improved contrast, thus making the test easier to interpret, yet did not significantly diminish the specific yellow-green fluorescence. Occasional crossreactions were observed with specimens from individuals with viral, bacterial, mycotic, and parasitic infections as well as with degenerative diseases. Although cross-reactions were frequently observed in individuals with mucocutaneous leishmaniasis, negative results were reported from L. tropica patients. Reliable testing for visceral leishmaniasis fluorescent antibodies was possible even when dried blood smears were used instead of serum.


Descriptors :   *LEISHMANIA , *FLUORESCENT ANTIBODY TECHNIQUES , REPRINTS , MICROSCOPY , DIAGNOSIS(MEDICINE) , IMMUNOLOGY , PARASITES , BLOOD SERUM , DOGS , GASTROINTESTINAL SYSTEM


Subject Categories : Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE