Accession Number : AD0450388


Title :   PURIFICATION AND CHARACTERIZATION OF STAPHYLOCOCCAL ENTEROTOXIN B


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Schantz, Edward J ; Roessler, William G ; Wagman, Jack ; Spero, Leonard ; Stefanye, David


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/450388.pdf


Report Date : Aug 1964


Pagination or Media Count : 17


Abstract : A method has been developed for the isolation of enterotoxin B with a purity greater than 99% and in yields of 50 to 60% from cultures of staphylococcus aureus. The method involves: (1) removal of the toxin from the culture with CG-50 resin, partly neutralized at pH 6.4, and elution with 0.5 M phosphate buffer, pH 6.8 in 0.25 M sodium chloride; (2) readsorption of the toxin on CG-50, partly neutralized at pH 6.8, followed by elution of the toxin with 0.15 M disodium phosphate; and (3) adsorption of the toxin oncarboxymethyl cellulose and chromatographic elution with a gradient phosphate buffer 0.02 M to 0.07 M at pH 6.8. The purified toxin was eluted between 0.035 M and 0.045 M phosphate. Elution of the toxin was followed by the absorbance at 277 millimicrons and by the Oudin serological test. Final preparations were assayed in rhesus monkeys. Ultracentrifugal and electrophoretic studies show that the purified toxin is a single component with a molecular weight of approximately 35,000. Analyses show that the toxin is composed of amino acids only and therefore is a single protein.


Descriptors :   *ENTEROTOXINS , *STAPHYLOCOCCUS AUREUS , PURIFICATION , IMMUNE SERUMS , IMMUNOLOGY , INFRARED SPECTRA , ELECTROPHORESIS , AMINO ACIDS , ION EXCHANGE RESINS , BACTERIAL TOXINS , CHROMATOGRAPHIC ANALYSIS , CULTURE MEDIA , CARBOXYMETHYLCELLULOSE , ULTRACENTRIFUGES


Subject Categories : Biochemistry
      Toxicology


Distribution Statement : APPROVED FOR PUBLIC RELEASE