Accession Number : AD0449641


Title :   A METHOD OF CHARACTERIZING PROTEIN CHANGES IN SERUM AFTER X-IRRADIATION,


Corporate Author : TORONTO UNIV (ONTARIO)


Personal Author(s) : Baker, D G ; Carsten, A L ; Safford, C


Report Date : 28 Feb 1963


Pagination or Media Count : 12


Abstract : A method of analyzing starch gel electrophoretic patterns was developed. Separation of the plasma proteins into well-defined groups was obtained by electromigration through a starch gel medium. Staining of these gels with Amido Black produces a characteristic pattern of protein fractions. By photographing these gels and evaluating the negatives with a recording densitometer, it was possible to gain a previously unattainable degree of definition. The resulting patterns were then analyzed by means of an expanding scale technique. This has been applied to the plasma proteins from groups of young and old rats, and to rats at various times after exposure to wholebody X-irradiation. The qualitative changes in the protein spectrum which occurred with age and irradiation included a general decrease in electrophoretic modility and an increase in the variability of the rate of electrophoretic migration of most fractions. A possible mechanism for these changes was discussed. (Author)


Descriptors :   *BLOOD PROTEINS , BLOOD , RADIATION EFFECTS , BLOOD SERUM , AMINO ACIDS , GLOBULINS , RATS , ELECTROPHORESIS , SPECTROSCOPY , DISTRIBUTION , ALBUMINS , BIOCHEMISTRY


Distribution Statement : APPROVED FOR PUBLIC RELEASE