Accession Number : AD0445242


Title :   CHROMATOGRAPHIC SEPARATION OF PHOSVITIN, ALPHA- AND BETA-LIPOVITELLIN OF EGG YOLK GRANULES ON TEAE-CELLULOSE


Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) DIV OF BIOLOGICAL SCIENCES


Personal Author(s) : Radomski, M W ; Cook, W H


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/445242.pdf


Report Date : 16 Apr 1964


Pagination or Media Count : 15


Abstract : The two components of lipovitellin and the three major components of yolk granules, phosvitin, alpha and beta-lipovitellin, were separated by gradient elution chromatography on TEAE-cellulose. A 0.2 M phosphate buffer (pH 6.8) had the necessary ionic strength to dissolve these proteins and when applied in this solvent all components except beta-lipovitellin were retained by the column. A linear gradient of ionic strength (limit buffer 0.2 M phosphate plus 0.5 M NaCl) was used to remove the other components. Recovery was essentially complete and the composition and properties of the individual components were similar to those obtained by previous chromatographic methods that gave only partial recovery.


Descriptors :   *PHOSPHOPROTEINS , *PROTEINS(CONJUGATED) , *LIPOPROTEINS , SEPARATION , EGGS , ULTRACENTRIFUGES , CHROMATOGRAPHIC ANALYSIS , ULTRAVIOLET SPECTRA , CELLULOSE , VISIBLE SPECTRA , REPRINTS , BUFFERS , PHOSPHATES


Subject Categories : Biochemistry


Distribution Statement : APPROVED FOR PUBLIC RELEASE