Accession Number : AD0443674


Title :   DEFINED MEDIUM FOR MAINTAINING CHICK FIBROBLAST MONOLAYERS


Descriptive Note : Technical manuscript


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Zebovitz, Eugene


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/443674.pdf


Report Date : Jun 1964


Pagination or Media Count : 14


Abstract : A defined maintenance medium that supports monolayers of chick fibroblast (CF) cells in a viable state and permits the formation of plaques by Venezuelan (VEE) and eastern (EEE) equine encephalitis viruses is described. The requirements for the individual constituents of a defined medium for maintaining CF monolayer viability and for plaque formation were determined by systematically omitting one component at a time from an otherwise complete medium. In the absence of amino acids, the monolayers died. Histidine monohydrochloride combined with L-cystine or L-cysteine were found to be the only amino acids necessary to maintain viable monolayers and permit plaque formation by VEE and EEE viruses. Any other amino acids, alone or in combination, failed to maintain the CF cell cultures. Glucose, the sole carbohydrate source, and sodium chloride were also necessary for cell survival.


Descriptors :   *CHICKENS , *BACTERIOPHAGES , *EQUINE ENCEPHALOMYELITIS VIRUS , *VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS , MAINTENANCE , PRODUCTION , SODIUM , VIABILITY , CHLORIDES , SURVIVAL(PERSONNEL) , AMINO ACIDS , GLUCOSE , ANTIBIOTICS , CULTURE MEDIA , HISTIDINE , VITAMINS


Subject Categories : Anatomy and Physiology
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE