Accession Number : AD0442462


Title :   THE METABOLISM OF ACETATE BY WHEAT STEM RUST UREDOSPORES,


Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB


Personal Author(s) : McConnell,W B


Report Date : 30 Dec 1963


Pagination or Media Count : 6


Abstract : When uredospores of Puccinia graminis var. tritici (race 15B) were incubated at pH 6.2 in phosphate buffer containing either acetate-1-C14 or 2-C14, about 12% of the radioactivity was removed from the solution in a period of 3 hours. Respired carbon dioxide contained about 45% and 22% of the carbon-14 taken up as acetate-1-C14 and acetate-2-C14, respectively. Incorporation of carbon-14 into spore components was considerably higher with acetate-2-C14 than with acetate1-C14. With either tracer most of the radioactivity in water-soluble spore materials was accounted for in amino acids and neutral substances. Glutamic acid was particularly radioactive and accounted for about 40% of the radioactivity in the amino acid fraction. Incorporation of carbon-14 into the glutamic acid skeleton was consistent with the view that both the tricarboxylic acid cycle and the glyoxalate cycle were functioning. (Author)


Descriptors :   *PUCCINIA(RUSTS) , *BIOCHEMISTRY , METABOLISM , ACETATES , SPORES , BIOASSAY , TRACER STUDIES , AMINO ACIDS , GLUTAMIC ACID , LABELED SUBSTANCES


Distribution Statement : APPROVED FOR PUBLIC RELEASE