Accession Number : AD0442407


Title :   PHOSPHATE LOSS BY RESTING E. COLI SUSPENDED IN SOLUTIONS OF SUCROSE OR SALTS


Descriptive Note : Technical manuscript


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Mercer, W Burtis ; Collins, Donald E


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/442407.pdf


Report Date : May 1964


Pagination or Media Count : 21


Abstract : Suspensions of resting Escherichia coli were prepared from cell thawed and washed after frozen storage and from freshly harvested cells. Washing fluids, phosphate-free, were water or 0.05 ionic strength salt solutions. After washing, the cells were resuspended in initially phosphatefree solutions of sucrose, inorganic salts, or sodium citrate. Phosphate from the cells immediately began to accumulate in the suspending fluid. The rate of accumulation was examined briefly and the effect of solute concentration and molecular type on extent of accumulation was examined more extensively. In the presence of extracellular sucrose the extent of phosphate accumulation in a given time period was a monotonically increasing function of solute concentration. In contrast, during the same time period the use of any ionic extracellular solute tested resulted in a minimum accumulation at a common specific ionic strength. Evidence was developed supporting the hypothesis that the (unidentified) phosphate involved established an equilibrium across the cell membrane.


Descriptors :   *METABOLISM , *ESCHERICHIA COLI , TEMPERATURE , SALTS , DETERMINATION , SUCROSE , PHOSPHATES , CELLS(BIOLOGY) , MEMBRANES(BIOLOGY) , SOLUTIONS(MIXTURES)


Subject Categories : Anatomy and Physiology
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE