Accession Number : AD0437967


Title :   RIFT VALLEY FEVER VIRUS ASSAY IN SERUM-FREE HAMSTER KIDNEY MONOLAYERS


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Boyle, John J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/437967.pdf


Report Date : Apr 1964


Pagination or Media Count : 10


Abstract : High titers of some arboviruses, including Rift Valley fever (RVF) virus, are obtained in cell cultures without any observable cytopathogenic changes. In the absence of serum, however, a rapid destruction of tissue-culture cells occurred with RVF virus. Preparation of virus dilutions in a serum-free maintenance medium and observation of infected tube monolayers has been found to be an effective method for achieving reproducible titrations of RVF virus. It is possible to calculate a median cytopathogenic effect dose (CPED50) comparable to titers determined by a plaque technique but more sensitive than in vivo titration. The tube monolayer titration is more economical, more rapid, and simpler to perform than previously used methods.


Descriptors :   *BIOASSAY , *RIFT VALLEY FEVER VIRUS , TISSUE CULTURE CELLS , TISSUE CULTURE , HAMSTERS


Subject Categories : Biochemistry
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE