Accession Number : AD0436047


Title :   FACTORS AFFECTING THE SAFE USAGE OF THE PURINE RIBOSIDES IN THE PRESERVATION OF HUMAN RED CELLS (PART I); THE INFLUENCE OF POPULATION DISTRIBUTION ON THE METABOLISM AND VIABILITY OF THE ERYTHROCYTE (PART II).


Descriptive Note : Final rept. 1 Mar 55-30 Jun 58,


Corporate Author : HARVARD MEDICAL SCHOOL BOSTON MASS


Personal Author(s) : Gibson,John G , II


Report Date : 01 Apr 1964


Pagination or Media Count : 69


Abstract : The effect of inosine added to ACD and CPD is an inhibition of glucose utilization accompanied by pentose breakdown with little change in lactate production. Ester phosphate is stabilized, but ion transport appears to be unaffected. Inosine breakdown is complete at 5 days of storage. In vivo studies in humans and rabbits show acceptable preservation of red cells up to 20 days of storage. Addition of inosine to ACD and CPD bloods at 14 and 21 days of prolongs the storage period to 20 days. There is a definite relationship to red cell age (by Fe59) and density (differential centrifugation), the youngest cells being the lightest. The uptake of Cr51 is determined by population distribution, being greatest in the youngest and progressively increasing with age. The extreme differential progressively on refrigerated storage. (Author)


Descriptors :   *METABOLISM , PRESERVATION , ERYTHROCYTES , BLOOD , VIABILITY , ADDITIVES , BIOCHEMISTRY , GLUCOSE , TRANSPORT PROPERTIES , STORAGE , PHOSPHATES , SURVIVAL(PERSONNEL) , ANTICOAGULANTS , LABELED SUBSTANCES , BLOOD TRANSFUSION , URIC ACID , AGING(PHYSIOLOGY)


Distribution Statement : APPROVED FOR PUBLIC RELEASE