Accession Number : AD0433665


Title :   THE CHEMICAL MODIFICATION OF NECROGENIC AND PROTEOLYTIC ACTIVITIES OF CROTALIDAE VENOM AND THE USE OF EDTA TO PRODUCE A VENOM TOXOID


Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY


Personal Author(s) : Goucher, Charles R ; Flowers, Herschel H


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/433665.pdf


Report Date : 09 Dec 1963


Pagination or Media Count : 29


Abstract : This report represents an investigation of the proteolytic activity, necrolytic activity, and antigenicity of untreated venom and venom which had reacted with ethylenediaminetetraacetic acid (EDTA). Agkistrodon piscivorus venom treated with EDTA lost ability to digest casein. Neither sodium phosphate nor sodium bicarbonate restored this ability. The hemorrhagic and necrotizing activity of this venom was essentially absent following EDTA treatment. EDTA treated venom which did not produce necrosis and hemorrhage stimulated the production of antibodies which neutralized the local and systemic toxic activity of untreated venom. The usefulness of EDTA in the treatment of snakebite should be investigated and attempts should be made to isolate and characterize the necrogenic and proteolytic components of snake venom.


Descriptors :   *BIOCHEMISTRY , *ANTIBODIES , *VENOMS , INHIBITION , ANTIGENS , TOXINS AND ANTITOXINS , NECROSIS , REPTILES


Subject Categories : Toxicology
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE