Accession Number : AD0432471


Title :   PURIFICATION AND LIPID CONTENT OF VEE VIRUS


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Wachter, Ralph F ; Johnson, Emil W ; Heydrick, Fred P


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/432471.pdf


Report Date : Mar 1964


Pagination or Media Count : 20


Abstract : The virus of Venezuelan equine encephalitis (VEE) was purified from chick embryo suspensions by two methods, and the total lipid content and RNA content of the purified virus preparations were compared. The lipid content of VEE virus purified by sucrose gradient centrifugration was much lower (24 per cent) than that of VEE virus purified without gradient centrifugation (54%). Virus of lower lipid was still fully infectious. With the decrease in lipid content of the virus there was a concomitant increase in RNA content. Electron microscopic examination of purified VEE irus showed that the virus had an average diameter of 68 millimicrons and possessed a center core about 30 millimicrons in diameter.


Descriptors :   *ELECTRON MICROSCOPY , *VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS , LIPIDS , PURIFICATION , VIABILITY , RIBONUCLEIC ACIDS


Subject Categories : Pharmacology
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE