Accession Number : AD0430795


Title :   STUDIES ON THE NATURE OF THE GENETIC MATERIAL TRANSFERRED DURING CONJUGATION IN ESCHERICHIA COLI.


Descriptive Note : Final rept. 20 Apr 62-19 Apr 63,


Corporate Author : KYOTO UNIV (JAPAN)


Personal Author(s) : Yura,Takashi


Report Date : 19 Apr 1963


Pagination or Media Count : 18


Abstract : Evidence suggests that cell walls are capable of specifically interacting with cells of opposite mating types and that these interaction are responsible for the observed inhibition of genetic conjugation. Futhermore, it is shown that cell walls of male or female bacteria contain a specific mating substance or substances. This represents the first evidence for the presence of a female specific substance. The cell-free information of the tryptophan synthetase was studied both from genetic and biochemical viewpoints. Crude extracts obtained from partially derepressed wild-type cells are able to form the enzyme under certain conditions. It was found that two distinct mechanisms exist, one of them being a partially DNA-dependent system while the other represents the activation-and-release of the enzyme from ribosomes. The latter mechanism seems to be one of the steps involved in the activity increase in the former system. Results of the detailed studies on the nature of these two systems are presented. (Author)


Descriptors :   *ENZYMES , *GENETICS , BIOSYNTHESIS , ESCHERICHIA , BIOCHEMISTRY , BACTERIAL EXTRACTS , MOLECULAR PROPERTIES , SEX , CELLS(BIOLOGY) , MEMBRANES(BIOLOGY) , SURFACE PROPERTIES , METABOLISM


Distribution Statement : APPROVED FOR PUBLIC RELEASE