Accession Number : AD0430389


Title :   GENETIC DETERMINANTS OF COLICIN PRODUCTION, PRINCIPALLY THEIR RELATION WITH BACTERIOPHAGES AND WITH THE F AGENT F FERTILITY.


Descriptive Note : Annual technical rept. no. 3 (Final), 1 Dec 6230 Nov 63,


Corporate Author : LIEGE UNIV (BELGIUM)


Personal Author(s) : Fredericq,Pierre


Report Date : 30 Nov 1963


Pagination or Media Count : 9


Abstract : Many recombinants obtained in crosses of wild strain E.coli K260, carrying V and B colicinogenic factors linked to an F agent, with Fstrains, carrying a deletion in the try region, are partial diploids, heterozygous for the try marker. They have been analyzed in conjugation and transduction experiments. Crosses of such partial diploids with F- strains carrying various mutations in the oys, try region reveal that the partial diploids are of the F'type, carrying an episome in which all the genes from the try region, including cysB and T1, are linked to an F agent and to col factors V and B. As all the known markers from the episome are transferred together without segregation. In transduction experiments, segregation of the markers was obtained. Only 2% of the transductants are colicinogenic; most of them receiving V alone; some B alone and very few V and B together. The latter are also the only ones which receive the F agent of the donor and are able to transfer at a high rate the col factors with the try genes to other strains. In transduction experiments to strains already carrying an F agent, the presence of the F agent greatly favors establishing the transduced episome. (Author)


Descriptors :   ESCHERICHIA , GENETICS , MICROORGANISMS , FERTILITY , BACTERIOPHAGES


Distribution Statement : APPROVED FOR PUBLIC RELEASE