Accession Number : AD0428400


Title :   GENETIC REPLICATION AND MACROMOLECULAR SYNTHESES IN TEMPERATE BACTERIOPHAGES.


Descriptive Note : Final technical rept.,


Corporate Author : BRUSSELS UNIV (BELGIUM)


Personal Author(s) : Thomas,R


Report Date : 30 Sep 1963


Pagination or Media Count : 14


Abstract : Superinfection experiments using chloramphenicol, or shift down to block protein syntheses, show that the early syntheses are not mutant-specific nor even species-specific. A number of conditional defective (suppressor sensitive, sus) mutants of lambda have been isolated and partly characterized by functional and recombinational analysis. The final purpose is to identify the genes involved in the early functions. Mixed superinfections of lambda-lysogenic cells with (a) a heteroimmune (434 hy) or strong virulent (lambda v) phage and (b) a normal lambda show that the control of genetic replication does not entirely depend on the repression of early protein syntheses. The genetic segment involved in lysogenization in phage 434 hy has been analysed and compared with the corresponding region in lambda. The genetic segments involved in the two aspects of immunity cannot be separated from each other or crossed between lambda and 434 hy. After superinfection of a lysogenic bacterium with a heteroimmune phage the progeny contains very few particles with genetic markers from the prophage. A high percent of them are partially diploid, being heterozygous for at least three loci. (Author)


Descriptors :   MICROORGANISMS , GENETICS , BACTERIOPHAGES , SYNTHESIS , MUTATIONS , PROTEINS , IMMUNITY


Distribution Statement : APPROVED FOR PUBLIC RELEASE