Accession Number : AD0428033


Title :   OBSERVATIONS OF A PHENOTYPIC MODIFICATION OF VARIOLA VIRUS IN TISSUE CULTURE


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Purlson, Elliott H ; Brown, Arthur


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/428033.pdf


Report Date : Dec 1963


Pagination or Media Count : 19


Abstract : An analysis was made to define the steps in the growth cycle of variola virus where the qualitative difference between cell-associated virus and supernatant virus is reflected. Thus far, results show that adsorption and/or penetration is greater in extent and occurs more rapidly with cell-associated virus than with supernatant virus. Further, cell-associated virus has a shorter lag phase, and is released into the medium more rapidly and to a significantly higher titer than supernatant virus. It is concluded that supernatant virus is because relatively little is released for infection of new cells, and what is released is of relatively poor infectious quality. The same observation was made with vaccinia virus, but the change from p+ to p- was more gradual.


Descriptors :   *VIRUSES , *VARIOLA VIRUS , MUTATIONS , INFECTIOUS DISEASES , GROWTH(PHYSIOLOGY) , TISSUE CULTURE , VACCINIA VIRUS


Subject Categories : Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE