Accession Number : AD0427169


Title :   RELATIONSHIP OF COLD UPON THE BIOLOGICAL PROPERTIES PECULIAR TO INFLUENZA VIRUS AND ITS CAPACITY FOR MULTIPLICATION


Corporate Author : NEW HAMPSHIRE UNIV DURHAM


Personal Author(s) : Metcalf, T G


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/427169.pdf


Report Date : Aug 1963


Pagination or Media Count : 10


Abstract : Alaskan ground squirrels were examined for their excretion of animal and bacterial viruses. Eighty-one separate fecal samples from 25 animals yielded negative findings for bacterial viruses reactive against five strains of Escherichia coli. Sixty-six individual fecal samples from 24 animals failed to show the presence of animal viruses capable of causing cytopathogenic effects in HeLa, Human Amnion and the LLMK2 strain of monkey kidney monolayers. The significance of the findings for animal viruses is presented. Two successful ground squirrel breedings were accomplished with five baby squirrels produced in each litter. Neuraminidase activity of influenza virus was demonstrated at 25 C. The amount of neuraminic acid split from substrate by enzyme action at 25 C was less than that observed at 37 C. Ovomucin obtained from fresh hens eggs was prepared and shown capable of serving as a suitable substrate for influenza neuraminidase.


Descriptors :   *INFLUENZA VIRUS , *COLD TOLERANCE , ENZYMES , ESCHERICHIA , RODENTS , MICROORGANISMS , GASTROINTESTINAL SYSTEM , TISSUE CULTURE , EXCRETION , HIBERNATION


Subject Categories : Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE