Accession Number : AD0426982


Title :   LOW TEMPERATURE PRESERVATION OF MOUSE LYMPHOCYTES WITH DIMETHYL SULFOXIDE,


Corporate Author : NAVAL RADIOLOGICAL DEFENSE LAB SAN FRANCISCO CALIF


Personal Author(s) : Ashwood-Smith,M J


Report Date : 06 Nov 1963


Pagination or Media Count : 22


Abstract : Dimethyl sulfoxide has been shown to protect a variety of animal cells from the damaging effects associated with cooling to, storage at, and thaw ing from very low temperatures. This paper de scribes the successful preservation of mouse lymphocytes at -196 C with dimethyl sulfoxide. Viability of preserved lymphocytes was assessed by several in vitro techniques wncluded phase-contrast microscopy, motility studies and protein synthesising activity. The biological assessment of fresh and frozen lymphocytes was demonstrated in a splenomegaly assay system in which lymphocytes from mice of a parental strain were injected into F sub 1 hybrids. The results of both in vitro and in vivo tests indicated that more than 50 percent of the lymphocytes were viable after storage at -196 C for three months. Dimethyl sulfoxide was shown to be superior to glycerol as a low temperature preservative agent for lymphocytes. (Author)


Descriptors :   *LYMPHOCYTES , PRESERVATION , CRYOGENICS , VIABILITY , BLOOD , MICE , SULFOXIDES , ALKANES


Distribution Statement : APPROVED FOR PUBLIC RELEASE