Accession Number : AD0421486


Title :   THE BACTERICIDAL ACTIVITY OF NORMAL GUINEA PIG SERUM AGAINST LISTERIA MONOCYTOGENES AND ITS INHIBITION BY A LISTERIAL CELL EXTRACT,


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : Silverman, Sidney J ; Elwell, Lynn


Report Date : Sep 1963


Pagination or Media Count : 20


Abstract : Normal guinea pig serum contains bactericidins active against Listeria monocytogenes. The listeriocidal activity of the serum did not increase after infection or after the injection of killed Listeria cells. It was destroyed by heating at 56 C for 30 minutes or by absorbing the serum with bovine serum albumin-antibovine serum albumin precipitate. The activity was not reduced by the addition of EDTA or heparin. Absorption of the serum with cells of Listeria, Bacillus subtilis, Staphylococcus aureus, and Escherichia coli reduced the listeriocidal acitivity. Plasma was bactericidal to a lesser degree than serum. The activity of both plasma and serum was inhibited by the addition of a crude cell extract of Listeria, the mortalityenhancing factor. Lysozyme was not implicated in the bactericidal system. It was suggested that the bactericidal activity of guinea pig serum might be due either to a beta lysin-like substance originating from plate-lets or leucocytes or to the presence of antibodies produced as a result of contact with other Gram-positive organisms such as B. subtilis or S. aureus, which possess antigenic substances cross reacting with Listeria. (Author)


Descriptors :   *CORYNEBACTERIACEAE , GERMICIDES , BLOOD SERUM , CELLS(BIOLOGY) , BACTERIA , TISSUE EXTRACTS , LABORATORY ANIMALS , BACILLUS SUBTILIS , STAPHYLOCOCCUS


Distribution Statement : APPROVED FOR PUBLIC RELEASE